141c: METHANOCOCCOIDES MEDIUM

Modifications: Replace trimethylammonium chloride with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under 100% N2 gas. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture.

Growth observed:

Growth conditions: anaerobic 78 °C